Riboflavin 5′-Phosphate Sodium
Riboflavin 5′-phosphate sodium (CAS 130-40-5), also called riboflavin sodium phosphate or FMN sodium, is the water-soluble form of vitamin B2 used in liquid supplements, beverages and other clear formulations.
Identity
What it is.
Riboflavin itself barely dissolves in water. Adding a phosphate group and forming the sodium salt gives a compound that dissolves readily, which is why riboflavin 5′-phosphate sodium is the form used in liquid vitamins, syrups, drops, shots and clear beverages.
Its assay is expressed differently from riboflavin. The USP monograph specifies the content as riboflavin, at 73.0% to 79.0% on the dried basis, because part of the molecule's weight is the phosphate and sodium. Our material is produced to that monograph; a recent batch assayed at 74.8%.
Also known as: Riboflavin sodium phosphate, Riboflavin-5′-phosphate sodium, FMN sodium, Flavin mononucleotide sodium, E101(ii).
- CAS number
- 130-40-5
- Molecular formula
- C₁₇H₂₀N₄NaO₉P (anhydrous)
- Molecular weight
- 478.33 g/mol (anhydrous)
- Appearance
- Yellow to orange-yellow crystalline powder, hygroscopic
- Solubility
- Soluble in water
- HTS heading
- 2936.23
- Country of origin
- China
Specifications
Specifications.
Every batch is tested against the specification below and shipped with a certificate of analysis reporting the result and method for each test.
Riboflavin 5′-Phosphate Sodium USP
For liquid supplements, beverages and other water-based formulations.
| Test | Specification | Batch result | Method |
|---|---|---|---|
| Description | Yellow to orange-yellow crystalline powder | Conforms | Visual |
| Identification | Positive | Positive | USP <191> |
| Assay, as riboflavin | 73.0% – 79.0% | 74.8% | USP |
| pH | 5.0 – 6.5 | 6.0 | USP <791> |
| Specific rotation | +38.0° to +43.0° | +38.1° | USP <781S> |
| Lumiflavin | NMT 0.025 | 0.008 | USP <857> |
| Free riboflavin | NMT 6.0% | 4.9% | USP <621> |
| Riboflavin diphosphates | NMT 6.0% | 4.9% | USP <621> |
| Free phosphate | NMT 1.0% | Conforms | USP |
| Loss on drying | NMT 8.0% | 3.46% | USP <731> |
| Residue on ignition | NMT 25.0% | 23.5% | USP <281> |
| Aerobic plate count | NMT 1,000 CFU/g | < 1,000 CFU/g | USP <2021> |
| Yeast and mould | NMT 100 CFU/g | < 100 CFU/g | USP <2021> |
| E. coli | Negative | Negative | USP <2022> |
Packaging and storage
- Packaging
- 25 kg drums
- Shelf life
- 2 years from manufacture
- Storage
- Airtight container, dry and protected from light
- Production
- Fermentation, at our plant in Xintai, Shandong
Applications
Where it is used.
Dietary supplements
Liquid multivitamins, B-complex drops, syrups, effervescent tablets and softgel fills.
Industry pageFunctional beverages
Clear ready-to-drink beverages, energy shots and drink mixes where riboflavin would not dissolve.
Industry pageFood fortification and premix
Liquid and instantized nutrient premixes; colour (E101(ii)).
Industry pageRegulatory
Regulatory status.
A summary for formulators and quality teams.
Food use in the United States
Riboflavin-5′-phosphate (sodium) is affirmed as GRAS as a nutrient supplement under 21 CFR 184.1697, when it meets Food Chemicals Codex specifications.
Compendial standards
Monographs exist in the USP, the Food Chemicals Codex and the European Pharmacopoeia (as riboflavin sodium phosphate). Our certificates of analysis cite the monograph and method each test was run to.
Documentation
Documentation, as standard.
Every shipment carries a certificate of analysis that names our manufacturing site, Xintai Jiahe Biotech Co., Ltd.. The full document pack is sent with your quotation, so your QA review can start straight away.
Also tested by Centre Testing International (CTI), an independent laboratory. The report is available on request.
- Certificate of analysis for the batch
- Specification sheet
- Safety data sheet (SDS)
- Test methods
- FSSC 22000, ISO 9001, HACCP and Halal certificates
- Allergen statement
- GMO statement
- Residual solvent statement
- Heavy-metal results
- Country-of-origin statement
- Supply-chain traceability statement
Questions
Riboflavin 5′-Phosphate Sodium: common questions.
What is riboflavin 5′-phosphate sodium?
It is the sodium salt of riboflavin 5′-phosphate, also known as flavin mononucleotide (FMN). It delivers vitamin B2 in a form that dissolves in water. Its CAS number is 130-40-5.
What is the difference between riboflavin and riboflavin 5′-phosphate sodium?
Both supply vitamin B2. Riboflavin is very slightly soluble in water and assays at 98% or more. Riboflavin 5′-phosphate sodium is soluble in water and contains 73% to 79% riboflavin by weight, so more of it is needed to deliver the same amount of vitamin B2. Dry products usually use riboflavin; liquids usually use the phosphate sodium salt.
Why is the assay 73% to 79% and not 98%?
The USP assay for riboflavin 5′-phosphate sodium is reported as riboflavin. The phosphate group, the sodium and the water of hydration make up the rest of the weight. A lot that meets the monograph is fully in specification at about 75%.
How much riboflavin 5′-phosphate sodium replaces 1 mg of riboflavin?
Divide by the assay. At a typical 75% riboflavin content, about 1.33 mg of riboflavin 5′-phosphate sodium supplies 1 mg of riboflavin. Use the assay on the certificate of analysis for the lot you are formulating with.
Is riboflavin 5′-phosphate sodium GRAS?
Yes. It is affirmed as GRAS for use as a nutrient supplement in food under 21 CFR 184.1697.
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